FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297782
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG753.3127153E-431.275238
CGAGTAG7550.029.8407753
CCGAGTA8500.029.2670762
CTACTCG6100.029.239922
CCCGAGT10950.028.3998661
GCTGGGA58100.028.1026699
AGGCGGA13150.027.840994
CGGAGGT10000.027.2094527
GCTACTC17250.027.041611
TACTCGG6650.026.821173
CCCGTTA1458.4713975E-725.9961151
GGCGGAG13550.025.9791325
TTGGGAG45700.025.8640826
GGAGGCT46900.025.209969
GTGCTGG38350.025.077277
GAGTAGC17750.024.3279364
CCCAAAG48350.024.1680531
GGGAGGC54200.024.1488658
AGTAGCT30000.024.0936225
GGAGGCG13550.023.9018882
Produced by FastQC (version 0.11.5)