FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15390135
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG105000.073.928313
CGAGTAG68450.073.437543
CTACTCG103850.072.968842
CCGAGTA74000.069.870572
GCTACTC253300.067.760121
GCTGGGA710050.066.4696049
GAGTAGC175800.066.291064
AGTAGCT294200.063.905215
GTAGCTG303300.062.4833156
TCGGGAG135200.061.612746
GGAGGCT595150.059.8702749
CTCGGGA132800.059.842855
CCCGAGT92900.058.246441
AAGTGCT497800.058.2113655
AAAGTGC492150.057.8096124
ACTCGGG134850.057.513044
TAGCTGG345400.056.9068347
AGTGCTG508000.056.4361726
CCCAAAG588350.056.3683661
GTGCTGG510500.055.9454427
Produced by FastQC (version 0.11.5)