FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740253
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14700.042.1909373
CCGAGTA15550.041.3943522
AGTAGCT56750.038.6656385
GAGTAGC37300.038.042784
GTAGCTG59100.036.7301646
CTACTCG12300.036.670522
TTGGGAG82750.035.998936
GCTGGGA104150.035.8188939
TCGCGTT1452.0008883E-1135.645025
TACTCGG12600.035.425473
TAGCTGG67000.034.0113337
CTCGCGT2100.033.5618674
CCCGAGT21650.033.426371
GGAGGCT79600.033.2902459
GCTACTC31050.032.387591
CGGAGGT17100.032.147457
GGGAGGC115200.031.200388
ACTTTGG73750.030.8351383
GCACTTT74650.030.216061
TTTGGGA81800.030.2138885
Produced by FastQC (version 0.11.5)