FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12554010
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG77600.084.982223
CCGAGTA81100.081.6863562
TACTCGG117350.076.369163
GAGTAGC192650.075.657094
CTACTCG116800.074.5629962
AGTAGCT313800.073.174245
GCTACTC271900.073.0927351
GCTGGGA770300.071.423219
GTAGCTG325750.070.851466
CCCGAGT102700.068.485451
GGAGGCT629850.064.320669
TCGGGAG148200.063.881756
AAGTGCT500900.063.4221425
TAGCTGG381000.062.723947
AAAGTGC493350.062.5556764
ACTCGGG144600.062.068894
CCAAAGT541100.061.4283032
CTCGGGA149050.061.3502165
CCCAAAG589300.061.0561561
TTGGGAG456900.060.741176
Produced by FastQC (version 0.11.5)