FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962929
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13100.046.7389873
CCGAGTA13800.045.3951232
AGTAGCT53800.044.4714555
GAGTAGC32400.043.7551084
GTAGCTG53400.043.3055156
CCCGAGT16150.042.5890351
GCTGGGA99000.041.866329
AAAGTGC47600.040.568054
TAGCTGG58600.039.6952937
AAGTGCT50800.038.2900775
CGGAGGT14900.037.2157827
CAAAGTG54950.037.113123
CCCAAAG62050.037.050621
CCAAAGT57300.036.6620642
AGTGCTG53200.036.5776
CTACTCG9900.036.1588862
TTGGGAG75500.035.5474556
TACTCGG10200.035.0930183
GGAGGCT69050.034.5044989
GTGCTGG56200.034.4502457
Produced by FastQC (version 0.11.5)