FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962929
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83380.281410725670443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA451.6085667E-552.2551294
CGAGTAG11800.047.8064043
CTACTCG11250.044.711382
AGTAGCT53250.044.489845
GTAGCTG54100.043.7662856
CCGAGTA13450.043.6892472
TACTCGG12150.043.3339773
TTGGGAG83850.042.693176
GAGTAGC33550.041.754494
GCTGGGA100600.041.072189
GCTACTC26800.040.379081
TAGCTGG60400.040.0432437
GCACTTT66850.039.4843371
CCCGAGT16600.038.547321
TTTGGGA82250.038.2903025
AAAGTGC49400.037.492874
GGAGGCT75700.037.339099
ACTTTGG71900.037.136953
CACTTTG71850.036.3776662
CCCAAAG61450.036.3693121
Produced by FastQC (version 0.11.5)