FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840248
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1252.3596476E-730.088165
ACTCGCG1452.6560883E-829.1803263
CCGAGTA9200.028.6230132
CGGAGGT7700.028.0835597
TTGGGAG48450.028.0426926
CTACTCG7950.027.8000662
AGTAGCT33350.026.501945
TACTCGG8200.026.3730053
CGAGTAG9650.026.3076553
CTCGCGT1807.48696E-926.1181954
CGCGAGT2007.876224E-1025.924131
GCTGGGA60450.025.8096169
GGAGGCT47200.025.4881159
GCTACTC18350.024.9159321
CTCGGGA9600.024.485815
CGCGTTG1351.4553621E-524.3769826
GTAGCTG35350.024.2045846
GGGAGGC59350.023.9917378
TTTGGGA49800.023.9783925
TAGCTGG40000.023.6222447
Produced by FastQC (version 0.11.5)