FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840248
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2000.037.70417494
GTAGCTG35050.032.0337566
AGTAGCT35500.031.2317925
GCTGGGA66600.031.1778379
CCGAGTA9450.030.8234182
TACTCGG9800.030.6813743
CTACTCG9650.029.697742
TAGCTGG40900.028.8302487
CGAGTAG8800.028.829163
TTGGGAG52100.028.6739396
CGGAGGT10550.028.498737
GCTACTC21450.028.0856321
GAGTAGC21200.027.9225834
CCCAAAG49450.026.2690491
GGAGGCT53800.026.0214779
GGGAGGC70700.025.7816268
CTCGGGA11950.025.553995
CCCGAGT13750.025.329731
CCCGTTA1501.1495304E-625.1015341
GTGCTGG37900.025.0386037
Produced by FastQC (version 0.11.5)