FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9607509
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG64000.085.4068153
CCGAGTA67750.082.930172
TACTCGG90850.080.4363253
CTACTCG90250.079.700952
GAGTAGC158150.078.9937744
AGTAGCT257300.077.995555
GCTACTC214050.075.891671
GTAGCTG270900.073.790666
GCTGGGA628500.072.298369
CCCGAGT81650.070.6431661
TAGCTGG305550.067.603047
AAGTGCT388350.067.1128165
GGAGGCT520100.066.92529
TTGGGAG392500.066.7859046
AAAGTGC386650.066.1580354
CCAAAGT416250.065.763232
TCGGGAG120500.065.521876
CTCGGGA116100.065.19265
ACTCGGG113250.064.7572864
CCCAAAG455400.064.1476441
Produced by FastQC (version 0.11.5)