FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190419
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18900.059.425833
CCGAGTA20800.057.1270642
AGTAGCT75300.056.2205165
GAGTAGC45250.056.1653634
GTAGCTG81050.051.9655276
CCCGAGT25800.050.019821
TTGGGAG120300.049.7333646
GCTGGGA159750.049.0794269
CGGAGGT25050.046.2733157
TAGCTGG95650.046.14117
CTACTCG19250.046.01462
GGAGGCT122050.044.065159
TACTCGG20650.043.9301763
GGAGGTT65700.042.7377558
GGGAGGC154000.042.636998
GCTACTC48300.041.794551
GCACTTT104150.040.3569261
TTTGGGA121700.039.873265
TGGGAGG154850.039.7527667
CAAGTAG39900.037.7125433
Produced by FastQC (version 0.11.5)