FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8312843
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG32850.061.6207543
CCGAGTA35750.060.9506952
TACTCGG40900.059.9358253
CTACTCG39850.059.650612
CCCGAGT47950.053.969291
GAGTAGC82600.053.1353344
GCTACTC102300.052.8320731
GCTGGGA292950.052.3198559
AGTAGCT135450.051.1405835
GGAGGCT240750.049.882119
GTAGCTG141850.049.3571976
TCGGGAG50650.048.5208366
CTCGGGA51150.047.0780645
TTGGGAG211250.046.6219756
TAGCTGG156700.046.298627
CGGAGGT53950.043.6015137
ACTCGGG56200.043.5088924
CCCAAAG242050.042.9953651
AAGTGCT200250.042.938255
AAAGTGC198300.042.861274
Produced by FastQC (version 0.11.5)