FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732184
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT753.2703846E-431.3436664
CCGAGTA10700.029.4444642
CGAGTAG9800.028.7853
GCTGGGA60450.027.8305059
TCGCGGG1057.41507E-526.8625567
GAGTAGC23050.026.5163334
GTAGCTG35850.026.0978626
CTACTCG7600.025.986532
AGTAGCT36000.025.5973285
CGTCGAG1301.0642119E-525.3815611
CCCGAGT14000.025.2520681
GTCGCGG950.001302540124.7449996
GGAGGCT45050.024.0963639
TACTCGG9050.023.897383
TTGGGAG43950.023.5344926
CTAAGCG1401.9251995E-523.5116232
GCTACTC20150.023.3931471
CGGAGGT8250.022.7924737
GGGAGGC54350.022.7400058
TAAGCGG1252.4192137E-422.567443
Produced by FastQC (version 0.11.5)