FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732184
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1755.456968E-1232.32930494
TACTCGG9600.029.3605753
CGAGTAG9850.029.0923063
AGTAGCT34350.028.7195175
AGGCGGA12850.028.5151754
CTACTCG9250.028.4406032
TCGCCGT4400.027.85953594
TTGGGAG47750.027.4477946
GTAGCTG34800.027.4027046
GCTGGGA64150.027.0945329
CGGAGGT10250.027.0398797
CCGAGTA11150.026.5434992
CTCGGGA10750.025.3456885
GAGTAGC24950.025.230094
GGGAGGC63550.024.6153098
CCCGAGT13300.024.4148271
GGAGGCT50600.024.3235749
GCTACTC22350.024.0039981
CCCAAAG45450.023.918521
GGCGGAG14200.023.8192845
Produced by FastQC (version 0.11.5)