FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3526503
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14200.043.3579033
CCGAGTA14800.042.8703882
ATTCGCG450.001014385341.7765734
TACTCGG11950.040.5092933
AGTAGCT59350.040.3863685
CCCGAGT17900.040.2387771
GAGTAGC38450.040.2147064
GTAGCTG61500.038.6688046
GCTGGGA105100.038.057749
CGCGATA250.001702860937.59891544-45
TAGCTGG68250.037.048027
CTACTCG12300.036.6818662
TTGGGAG86700.036.0485576
GGAGGCT77850.033.14599
GCTACTC29400.032.1851161
GGGAGGC108100.032.129518
GCACTTT70750.032.0720671
TTTGGGA85150.031.2956315
CCCAAAG65600.031.0017571
TCGGGAG17000.030.9638146
Produced by FastQC (version 0.11.5)