FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650976
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10750.041.5703053
TACTCGG7100.041.0772553
CCGAGTA11100.040.261812
AGTAGCT38550.039.4121175
GAGTAGC25100.038.6051374
CTACTCG7050.038.0345572
CCCGAGT13450.037.1145171
GTAGCTG40500.037.018486
GCTGGGA73200.036.3389059
TTGGGAG57050.035.8357476
GCTACTC17550.034.8840141
TAGCTGG45050.034.531557
GGAGGCT53650.033.813039
TCGGGAG11150.033.2992976
GGGAGGC74150.030.9308288
AAAGTGC36350.030.6687164
CCCAAAG52200.030.4032251
TTTGGGA57500.030.2681265
AAGTGCT38650.029.2087765
GCACTTT50800.029.1089251
Produced by FastQC (version 0.11.5)