FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788296
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6350.033.3149953
CCGAGTA9200.032.706352
GCTGGGA66750.032.2453549
CGAGTAG8600.031.7051983
CTACTCG6200.030.3325022
AGTAGCT35950.030.0766815
GTAGCTG37400.029.4133976
TAGCTGG40400.029.203767
GGAGGCT49450.029.1759519
TTGGGAG53150.029.0115876
CGGAGGT8150.028.837587
GCTACTC18700.028.7256811
GAGTAGC23950.027.2841384
GGGAGGC64200.027.0844278
CCCGAGT13100.026.9772131
AAAGTGC38050.026.9341184
AAGTGCT40250.025.9291365
GTGCTGG40900.025.2847
GCACTTT47200.024.85791
CCCAAAG49500.024.8451961
Produced by FastQC (version 0.11.5)