FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788296
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT558.839852E-751.2523235
CCGAGTA8050.037.351592
CTACTCG7450.036.5760422
CGAGTAG7450.036.5760423
TTGGGAG52750.034.823436
TACTCGG8600.034.416533
GGAGGCT53100.033.1782849
GCTGGGA67500.032.9211169
CCCGAGT12000.032.543251
CGGAGGT11150.032.44397
AGTAGCT35100.031.8562625
CTCGCGT1351.3338649E-831.3208644
GAGTAGC22400.031.0412144
GTAGCTG36850.030.5978536
TAGCTGG40550.029.3120987
GGGAGGC69950.029.2158628
GCTACTC21900.028.1443541
CCCAAAG50450.027.791961
AGGCGGA15300.027.6360574
GGAGGTT30150.027.5805648
Produced by FastQC (version 0.11.5)