FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322702
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8950.036.2487263
CTACTCG9000.034.480072
CGGAGGT10600.031.9162757
CGTAACG600.004138601531.369611
TTGGGAG54800.031.2099676
GGAGGCT54900.030.7284289
AGTAGCT38700.030.3726465
GCTACTC22350.030.1064031
GTAGCTG39250.029.8079536
GGGAGGC69900.029.1732678
GCTGGGA69850.028.6590259
CGAGTAG11700.028.532463
TAGCTGG43600.027.0503437
GGCGGAG16450.026.8667775
AGGCGGA16050.026.6583374
GAGTAGC25250.026.628174
CCGAGTA12550.026.2253322
GGAGGTT31000.026.221468
CTCGGGA12050.025.7519725
TGGGAGG72450.025.4233767
Produced by FastQC (version 0.11.5)