FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789471
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT901.3116733E-841.789585
TACTCGG8100.036.5658843
CGAGTAG8650.036.414913
CCGAGTA10200.034.5735472
GAGTAGC21950.033.626814
CTACTCG8400.033.025972
TTGGGAG55700.032.8333326
CGGAGGT10750.032.3579867
GTAGCTG36950.032.0631836
AGTAGCT37350.031.593935
GGAGGCT51850.031.178219
GCTGGGA69650.031.1043629
GCTACTC19000.030.7673151
TAGCTGG40950.030.648957
CCCGAGT12750.029.9499681
GGGAGGC70400.028.6369068
CGCGAGT1503.5937774E-828.2860811
CCCAAAG48600.026.966841
CGGGTTC7900.026.7686759
TTTGGGA57400.026.7009225
Produced by FastQC (version 0.11.5)