FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780073
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1055.0749804E-835.843395
CTACTCG9050.033.7933042
TACTCGG9600.033.3244743
AGTAGCT48600.031.943765
GCTGGGA87900.031.4375389
CCGAGTA14800.030.519362
CGAGTAG13500.029.9702473
GTAGCTG50350.029.7781166
TTGGGAG71350.029.2479236
GCTACTC24250.028.9378471
CGGAGGT12300.028.6575627
GGAGGCT64450.028.2194239
GAGTAGC33350.028.2125644
TAGCTGG56950.028.0587447
GGGAGGC87650.027.2392318
CCCGAGT17600.027.027161
GGAGGTT36450.026.1746948
GCACTTT63000.025.417341
CCCAAAG65750.025.1421871
CTCGGGA12500.024.4631145
Produced by FastQC (version 0.11.5)