FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780073
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11800.041.8297353
TCGCGTT952.1162123E-839.5851825
CTACTCG11500.039.2422
CCGAGTA11900.039.1080362
CGAGTAG11850.036.496173
AGTAGCT48800.034.292295
GCTGGGA87100.034.043479
CGGAGGT15350.033.9774867
GCTACTC28900.033.8623471
TTGGGAG75100.033.8462836
GAGTAGC29100.033.43874
GGAGGCT74500.033.1781439
GTAGCTG49750.033.1487926
CTCGGGA14500.031.1221435
TAGCTGG56350.031.0188487
CCCGAGT16300.030.885011
GGGAGGC98500.030.3395428
TCGGGAG15900.029.2544356
TGGGAGG98400.027.7910237
ACTCGGG18100.027.5295644
Produced by FastQC (version 0.11.5)