FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123311
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10250.043.1168982
TACTCGG11400.041.2418983
TTGGGAG66150.040.9804236
CCGAGTA12300.040.8998952
AGTAGCT42750.040.137615
GCTACTC26150.039.7647441
GAGTAGC28200.039.6799744
CGAGTAG12250.039.5316243
CCCGAGT15100.038.3270871
GGAGGCT70150.037.7151229
GTAGCTG45550.037.2348256
CTCGGGA13550.036.0818565
GCTGGGA82050.035.8534169
TAGCTGG49200.035.814537
TCGGGAG15650.035.4240466
TTTGGGA64150.033.4166565
GGGAGGC94700.032.7993668
GCACTTT57900.032.018081
ACTCGGG15150.031.0335084
ACTTTGG60750.030.6473143
Produced by FastQC (version 0.11.5)