FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9723115
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG57950.080.485833
CCGAGTA61450.078.6616742
TACTCGG81850.075.713233
CTACTCG80450.075.490192
GAGTAGC139150.075.429244
GCTACTC187900.073.471021
AGTAGCT228550.073.039565
GCTGGGA540200.072.1292659
GTAGCTG235750.071.169326
CCCGAGT75250.066.5858461
AAGTGCT341600.066.493625
GGAGGCT449050.065.589859
AAAGTGC342600.065.461424
TAGCTGG267700.064.72227
CCAAAGT376400.064.245082
CCCAAAG410000.063.905321
TTGGGAG361250.063.8886266
CTCGGGA97100.063.6209075
AGTGCTG356900.062.9491926
TCGGGAG104400.062.6720666
Produced by FastQC (version 0.11.5)