FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301501
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG11100.044.049743
AGTAGCT52150.043.9938435
GTAGCTG52700.043.7791146
CGAGTAG14150.042.861393
CTACTCG11700.042.192612
CCGAGTA14400.042.1172682
GCTACTC27950.041.747651
GAGTAGC34250.041.7297674
TTGGGAG83150.041.592186
TAGCTGG57450.041.0641067
GCTGGGA97150.040.2478269
CCCGAGT17500.038.4467961
GCACTTT70250.038.176021
GGAGGCT80000.038.0081029
TTTGGGA81900.036.3941655
ACTTTGG74000.036.2139663
GGGAGGC112800.035.8709458
CACTTTG78050.034.8167232
AAAGTGC50250.034.430614
CTTTGGG82850.034.1047554
Produced by FastQC (version 0.11.5)