FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072359
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87990.286392312877499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT52400.043.8706635
CGAGTAG14300.043.7260933
CCGAGTA14950.043.710372
TACTCGG10350.043.606973
TCGCGTT555.278073E-542.7369965
CCCGAGT17100.042.6462671
CTACTCG10700.042.618562
GAGTAGC33150.042.5457044
GTAGCTG53300.042.128626
GCTGGGA94950.041.347169
TTGGGAG78650.039.8385626
TAGCTGG59000.039.7997937
GCTACTC27850.039.024041
GGAGGCT74750.037.0659569
GCACTTT65050.036.669591
CCCAAAG59700.036.6457861
CGGAGGT15550.035.6380927
AAAGTGC47800.035.2105754
TTTGGGA76600.035.165965
CACTTTG69400.034.8188552
Produced by FastQC (version 0.11.5)