FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9109027
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG35700.064.03493
CCGAGTA36950.063.986962
TACTCGG41750.060.0595473
CTACTCG41350.059.22592
GCTGGGA334800.058.4789479
GAGTAGC94800.056.66644
CCCGAGT45550.055.8250581
AGTAGCT160600.055.3432585
GTAGCTG162100.054.8731966
GCTACTC110000.053.642481
GGAGGCT270350.052.9732369
CGGAGGT56900.050.658737
TAGCTGG180650.050.6059077
TCGGGAG55150.048.4921076
CCCAAAG289050.048.4071661
AAAGTGC233950.048.150754
GTGCTGG233500.046.898627
TTGGGAG232200.046.7701646
AAGTGCT243550.046.493165
CTCGGGA53950.046.467245
Produced by FastQC (version 0.11.5)