FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9109027
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA37000.071.96512
CGAGTAG36250.070.2221153
TACTCGG48450.065.439553
CTACTCG49450.062.5356522
GCTGGGA333800.061.4724359
CCCGAGT48450.061.2905121
GAGTAGC92250.060.762714
GCTACTC120300.059.707941
AGTAGCT155250.056.1490175
GGAGGCT281950.055.3502859
GTAGCTG156950.054.7397966
TCGGGAG61600.052.407266
CCCAAAG295450.052.0837141
AAAGTGC240700.052.0390474
TAGCTGG173850.051.809737
ACTCGGG60200.051.8011474
CTCGGGA59600.051.666385
AAGTGCT245600.051.47715
AGTGCTG243650.051.182286
GTGCTGG243500.050.647757
Produced by FastQC (version 0.11.5)