FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921152
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10200.041.0174143
CCGAGTA11050.037.438092
GAGTAGC26600.036.5819784
AGTAGCT39700.036.4702455
CCCGAGT13000.035.8781931
GTAGCTG41650.034.0855566
GCTGGGA73850.033.790049
TTGGGAG63550.031.7336626
CGGAGGT10400.030.7322657
TAGCTGG48500.030.5272567
CGGGTTC6650.028.9738929
CCCAAAG52800.028.5532021
GGAGGCT56050.028.4229229
GGGAGGC75950.028.276988
CCGGGTT6500.028.1965438
TACTCGG9750.027.9641653
GCACTTT54800.027.8550031
AAAGTGC41700.027.8444964
CTACTCG9200.027.0820392
TTTGGGA64050.026.9355225
Produced by FastQC (version 0.11.5)