FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831583
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79750.2816445783153805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1256.2391337E-933.8593294
CCGAGTA10150.033.346482
CGAGTAG9650.033.1268843
CTACTCG8000.029.3807962
CTCGCGT1602.1318556E-929.3807964
GCTGGGA65550.029.3703049
TACTCGG9050.029.0896473
GAGTAGC22850.029.007914
GTAGCTG36450.028.9958976
TCGCGTT1303.329278E-728.9282675
AGTAGCT36850.028.7025745
CGGAGGT10150.028.6864597
TTGGGAG56850.028.5888846
GGAGGCT52550.028.0578389
CCCGAGT14200.027.1679381
GGGAGGC69650.027.1020558
TAGCTGG41300.027.0631397
ACTCGCG1406.3608786E-726.8633983
GCTACTC21650.026.294121
GCACTTT46550.024.4583381
Produced by FastQC (version 0.11.5)