FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12060760
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT4500.061.0412945
TACTCGG43500.053.9052663
CTACTCG44200.051.7034532
CCGAGTA38750.050.8467372
CGAGTAG36050.048.8768123
GCTACTC116300.048.8208541
CGGAGGT63650.047.4694637
GCTGGGA343200.046.377999
GGAGGCT280350.046.2373779
CCCGAGT54950.045.0685271
GAGTAGC95150.042.4581344
CTCGGGA54500.042.2877245
TCGGGAG57400.042.0187346
TTGGGAG250050.041.368796
CTCGCGT6850.041.0781674
AGTAGCT164350.040.1938485
GTAGCTG165300.040.003386
GTGCTGG247550.039.2681667
GCGGAGG78700.039.159356
GGCGGAG94400.038.6791465
Produced by FastQC (version 0.11.5)