FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9605382
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG45200.075.294453
CCGAGTA48750.071.308832
TACTCGG58200.070.990453
GAGTAGC121350.070.2632754
CTACTCG60200.067.315272
GCTGGGA460900.066.464429
GCTACTC147600.066.201491
AGTAGCT199400.066.132395
GTAGCTG204300.064.4478766
CCCGAGT59800.060.637721
GGAGGCT357600.060.0758179
TAGCTGG232350.058.9728787
AAAGTGC302600.058.1922384
CCCAAAG363800.057.2168121
AAGTGCT316550.057.0495385
TCGGGAG77500.056.175656
CCAAAGT337050.055.7540932
AGTGCTG316150.055.647946
GTGCTGG314350.055.24987
CTCGGGA74200.054.5114025
Produced by FastQC (version 0.11.5)