FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826222
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12550.042.706493
AGTAGCT46700.041.2762035
CCGAGTA13250.041.1621362
GTAGCTG47150.040.1842736
GAGTAGC31150.039.996374
GCTGGGA82950.037.2224549
CCCGAGT17300.036.5096321
TAGCTGG51950.036.1055537
CTACTCG8400.034.70312
TTGGGAG63950.033.1565066
TACTCGG8550.032.9927063
CGGAGGT12450.032.850097
GGAGGCT60400.030.1113369
AAAGTGC41450.030.0575854
CCCAAAG56450.029.7258851
GCACTTT54600.029.6108061
AAGTGCT42850.029.4046975
AAGTAGC23850.029.3718054
GGAGGTT33050.029.2921858
GCTACTC24050.029.0065041
Produced by FastQC (version 0.11.5)