FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7341700
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA24850.065.7252662
CGAGTAG24000.061.6983153
TACTCGG31950.057.8829573
CTACTCG32450.056.403162
GCTACTC81000.056.1044041
CCCGAGT36800.053.195981
GAGTAGC63300.052.5081334
GGAGGCT198850.052.157919
CGGAGGT45400.051.9330377
GCTGGGA231050.051.765369
AGTAGCT110900.049.6266485
GTAGCTG112100.048.8119476
TCGGGAG41100.047.9345326
TTGGGAG179250.046.587016
CTCGGGA39250.046.3078885
TAGCTGG122550.046.153267
CCCAAAG199550.044.5596661
GTGCTGG160500.042.9219937
AAAGTGC165500.042.892744
GCGGAGG57400.042.7376
Produced by FastQC (version 0.11.5)