FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15302170
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG77500.066.8106463
CTACTCG78000.066.120682
CGAGTAG54750.064.622223
GCTACTC193950.063.093091
CCGAGTA59600.062.9025152
GCTGGGA572900.059.6425639
GAGTAGC150550.058.6167684
TCGGGAG96250.055.8450626
CCCGAGT75450.055.6554641
AGTAGCT252400.055.1542435
GGAGGCT466000.054.402069
GTAGCTG255850.054.2770236
CTCGGGA99100.053.3470045
TAGCTGG281600.051.440067
GTGCTGG412500.051.330337
ACTCGGG101350.050.887154
AAAGTGC424700.050.657334
CCCAAAG512750.050.3187681
AGTGCTG425650.050.192116
AAGTGCT436300.050.0434465
Produced by FastQC (version 0.11.5)