FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20711263
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG89350.069.65533
CCGAGTA95350.067.313892
TACTCGG131950.065.8775943
CTACTCG132800.065.114992
GCTACTC325550.060.6180081
GCTGGGA913350.058.7725569
GAGTAGC228750.058.713944
TCGGGAG167950.056.3018656
GGAGGCT753150.055.952649
CCCGAGT126650.055.3652571
AGTAGCT384500.054.9445955
GTAGCTG387750.054.2534986
CTCGGGA168400.052.6802375
AAAGTGC637650.051.6490754
ACTCGGG168100.051.5469444
AAGTGCT656300.051.1034745
CCCAAAG773700.050.3804241
GTGCTGG665300.049.8223657
AGTGCTG668350.049.7396556
TAGCTGG445000.049.5448347
Produced by FastQC (version 0.11.5)