FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9258983
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG61500.082.320613
TACTCGG88300.079.484363
CCGAGTA64400.078.250032
CTACTCG86950.078.203392
AGTAGCT244550.077.379065
GAGTAGC155250.076.932864
GCTACTC208250.076.152441
GTAGCTG255800.074.794226
GCTGGGA591150.073.7917949
TAGCTGG285600.069.051297
AAGTGCT369250.067.703775
GGAGGCT494600.067.340969
CCCGAGT75650.067.280021
AAAGTGC366800.066.9255454
TTGGGAG360500.066.534726
CCCAAAG425200.066.121161
CCAAAGT393000.065.678622
TCGGGAG116150.064.480596
ACTCGGG108700.063.8452384
CTCGGGA112750.063.8144265
Produced by FastQC (version 0.11.5)