FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9258983
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG59800.086.84163
CCGAGTA63850.082.767332
GAGTAGC152600.079.7627944
AGTAGCT238000.079.749695
TACTCGG90450.079.396673
CTACTCG89650.078.719022
GCTACTC203400.078.117091
GTAGCTG249300.075.610586
GCTGGGA584500.074.67989
AAGTGCT365150.070.590075
AAAGTGC361600.070.071234
CCCGAGT77400.068.96341
GGAGGCT493650.068.828439
TAGCTGG282750.068.630387
TTGGGAG376550.068.0017246
CCAAAGT392850.067.8769762
CCCAAAG428950.067.053161
TCGGGAG116000.065.968826
CAAAGTG394450.065.860623
AGTGCTG382800.065.7808766
Produced by FastQC (version 0.11.5)