FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168285
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91960.29025166612220804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT901.31276465E-841.7860225
CCGAGTA10800.032.652
CGAGTAG10800.032.648443
GAGTAGC23600.030.87674
AGTAGCT38950.030.0521435
CCCGAGT14300.029.9430581
CGCCGTA1303.3174183E-728.94027794
TACTCGG9250.028.4623813
TTGGGAG53800.028.3797656
GGAGGCT54200.028.3306339
GTAGCTG39150.028.0796576
ACTCGCG1354.6271634E-727.8600043
GGAGGTT31550.027.5346898
CTACTCG8750.027.403612
CGGAGGT11200.027.2557587
GCTGGGA69300.026.9685549
CTCGGGA10350.026.3433635
GAGGTTG39100.026.3011769
GCTACTC22450.026.1989841
GGGAGGC70350.026.1655458
Produced by FastQC (version 0.11.5)