FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15997888
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG79050.069.586593
CTACTCG79400.068.162442
CGAGTAG59300.066.752223
CCGAGTA64050.064.8919142
GAGTAGC146850.061.3003924
GCTGGGA560700.060.713939
CCCGAGT78900.058.745711
TCGGGAG100200.055.920096
CTCGGGA99100.055.645515
AGTAGCT250400.055.1928865
GGAGGCT459000.055.044789
GTAGCTG256400.052.8632286
ACTCGGG105100.052.403864
TAGCTGG277550.051.1709257
GTGCTGG413250.051.0644957
CGGAGGT109400.050.7807967
AAAGTGC433200.049.79994
AGTGCTG429450.049.7579966
CCCAAAG512150.049.222711
AAGTGCT444750.048.629385
Produced by FastQC (version 0.11.5)