FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9930131
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG62850.074.5189743
CTACTCG64100.072.255842
CGAGTAG42700.070.0338443
CCGAGTA45050.066.668232
GCTGGGA439800.062.877799
GAGTAGC114100.062.7085844
AGTAGCT189150.061.3880425
GGAGGCT375800.059.604219
TCGGGAG81800.058.8420376
CTCGGGA78800.058.6125875
GTAGCTG196950.058.29816
TTGGGAG302850.058.025456
AAAGTGC287300.057.440554
CCCGAGT59300.056.4529721
AAGTGCT295900.056.0122575
TAGCTGG215200.055.2230457
ACTCGGG84300.054.7885174
CCCAAAG351650.054.4993931
AGTGCTG297400.054.3337756
CCAAAGT322800.054.1574252
Produced by FastQC (version 0.11.5)