FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399331
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG30050.057.526723
CTACTCG30150.053.5289042
CGAGTAG25150.051.7412573
GCTACTC81900.051.157421
CCGAGTA27250.050.095932
GCTGGGA236450.049.068559
CGGAGGT41400.048.7855877
GGAGGCT197250.048.419169
CTCGGGA37650.047.0886155
GAGTAGC67450.046.8017354
AGTAGCT113600.045.07895
GTAGCTG113600.044.311096
CCCGAGT38050.043.3065641
TCGGGAG41300.043.198796
TTGGGAG178150.042.9199336
TCGCGTT3000.042.515265
TAGCTGG126250.042.3411987
CCCAAAG215650.041.2859231
GTGCTGG164750.040.8772967
GGGAGGC232050.040.8009078
Produced by FastQC (version 0.11.5)