FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8399331
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG31650.061.8472443
CGAGTAG25950.059.2154923
CTACTCG31650.059.027552
CCGAGTA27950.057.0320632
GCTACTC84350.055.0454181
GCTGGGA241700.054.7116139
CGGAGGT48150.053.2106937
GAGTAGC66850.051.599914
CCCGAGT37100.051.2291641
GGAGGCT204500.051.1079649
CTCGGGA39150.049.020475
AGTAGCT120450.047.3760645
GTGCTGG167550.047.015737
GTAGCTG121900.046.5899626
ACTCGGG43550.046.1185344
TCGGGAG43450.046.0636376
TAGCTGG132350.045.458727
CCCAAAG224350.045.11551
TTGGGAG184900.044.6083076
AAAGTGC184650.043.5085224
Produced by FastQC (version 0.11.5)