FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2925055
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11350.030.2651312
CCCGAGT14550.030.1136671
CCCGAAT5950.029.2973561
TACTCGG9250.027.9773643
CGAGTAG10800.027.8831673
GCTACTC20550.027.7406881
CTACTCG8550.027.5181642
TTGGGAG45300.027.3901186
AGTAGCT33400.026.9060825
GTAGCTG34350.026.4069796
GCTGGGA58950.026.1486029
CTCGCGT1301.0795617E-525.3348454
TAGCTGG39450.024.898047
TCGCGTT950.001296937224.7633825
GGAGGCT47900.024.724279
GGGAGGC58750.024.3182098
GAGTAGC23150.024.185754
CTCGGGA10600.023.9690865
CGGAGGT8200.023.498277
TGGGAGG60750.022.5892837
Produced by FastQC (version 0.11.5)