FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2925055
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1405.0931703E-1033.582395
CGAGTAG9550.033.478613
CGCCGTA1755.456968E-1232.2291794
CCGAGTA10400.030.742382
TTGGGAG50400.028.900376
CGGAGGT11700.028.515077
GCTGGGA61400.028.3937249
GCTACTC20650.028.0282671
TACTCGG9450.027.8623753
AGTAGCT36300.027.5875155
ACTCGCG1555.070433E-827.3006343
GTAGCTG36700.026.7579296
AGGCGGA15700.026.6533784
TCGGGAG11500.026.5575376
GGAGGTT28500.026.5450158
TAGCTGG38800.026.5224937
CTCGCGT1606.8983354E-826.4474874
GGAGGCT50750.026.2039769
GGCGGAG16450.026.0084885
CTACTCG9700.025.6901152
Produced by FastQC (version 0.11.5)