FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223833
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12200.032.4251983
AGTAGCT46100.031.9747075
GTAGCTG45200.031.817266
CCGAGTA12750.031.400222
TTGGGAG64600.031.0652716
GCTGGGA78950.030.8921329
GAGTAGC28850.030.6884674
TACTCGG9950.029.3448453
CCCGAGT16000.028.8556791
CCGAACG1154.130354E-628.6697652
CGGAGGT12550.028.4578787
TAGCTGG51250.028.2416137
TCGCGTT856.739814E-427.70225
GGAGGCT60700.027.328689
CTACTCG10100.026.5813712
GGGAGGC79850.026.5420388
GGTTCGT1956.0754246E-1026.5088398
GCTACTC22350.025.716241
TGGGAGG82400.024.808177
AGGCGGA16750.024.4606294
Produced by FastQC (version 0.11.5)