FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14129719
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG58750.077.976513
CTACTCG96850.077.77682
TACTCGG97500.077.442243
GCTACTC240550.071.7382661
CCGAGTA65250.071.70532
GAGTAGC155900.067.347934
GCTGGGA653000.066.801219
TCGGGAG121600.065.985356
GGAGGCT551500.063.5400479
AGTAGCT259700.063.218965
CTCGGGA123000.062.725285
ACTCGGG123250.060.8174864
CCCGAGT83000.060.5856251
GTAGCTG273400.059.7249876
AAGTGCT475700.059.7134445
AAAGTGC467050.059.6740534
CCCAAAG569000.058.193091
CCAAAGT519550.057.7562262
GTGCTGG479100.057.2993937
AGTGCTG488500.057.095896
Produced by FastQC (version 0.11.5)