FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264410
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8700.036.2304762
CCGAGTA12100.035.770172
TACTCGG9150.034.9606673
CGAGTAG11000.033.3575253
TTGGGAG59450.032.808686
GCTACTC21200.031.7588751
AGTAGCT37850.031.070345
GCTGGGA70250.030.773549
GAGTAGC24800.030.1590274
GTAGCTG38400.029.8642186
GGGAGGC75650.029.8207238
GGAGGCT56050.029.5143369
CGGAGGT11800.029.4737647
CCCGAGT15500.029.1611371
ATGCGCG650.00612995728.9479984
TCGGGAG12200.028.1226066
TGGGAGG75650.027.5841677
GCACTTT55100.026.7459281
AGGCGGA15450.026.1843554
TAGCTGG45500.026.1333487
Produced by FastQC (version 0.11.5)