FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809205
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT39750.036.3054125
TTGGGAG54750.035.3739286
CTACTCG8250.035.329612
GAGTAGC25800.034.6181684
GTAGCTG41250.034.1875086
GCTGGGA74450.034.1490829
TACTCGG8000.033.4930763
TAGCTGG46150.032.9993067
CGAGTAG10750.032.7961583
CCGAGTA11050.032.7589152
GGAGGCT57650.032.0361029
GCACTTT48850.030.2875981
GGGAGGC75100.029.9112428
CCCGAGT14100.029.7420311
GCTACTC20750.028.1580981
TGGGAGG76300.027.7832817
TTTGGGA56050.027.6763295
CCCAAAG49200.027.5820881
ACTTTGG51550.027.2654513
AAGTGCT39750.026.4899435
Produced by FastQC (version 0.11.5)