FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834165
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1001.2732926E-1147.0912135
CCGAGTA9100.033.122612
CGGAGGT9500.030.6692377
CTCGCGT1401.8577339E-830.2729244
GCTGGGA60650.029.9857569
TTGGGAG50200.029.30576
CGAGTAG9350.029.2116623
CGCGTTG1452.6622729E-829.1733286
AGTAGCT34700.028.9061345
GAGTAGC22450.028.5274144
ACTCGCG1503.6327037E-828.2547263
TACTCGG8400.028.0304853
GTAGCTG35300.027.8280666
GGAGGTT25800.027.1394738
CCCGAGT13050.027.0710951
GGAGGCT49300.026.9758019
GGGAGGC62250.026.8748158
TAGCTGG39350.026.8703087
GCTACTC20300.026.6843641
CTACTCG8200.026.4198272
Produced by FastQC (version 0.11.5)