FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8818312
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG47100.076.76863
TACTCGG65450.076.409343
CTACTCG65300.075.726752
CCGAGTA51500.074.288062
GAGTAGC120850.071.032484
AGTAGCT201950.070.168655
GCTACTC152900.069.6407551
GCTGGGA472450.068.825039
GTAGCTG208500.067.527646
TCGGGAG84700.062.8825576
GGAGGCT387750.061.301039
TAGCTGG236450.061.250647
ACTCGGG79300.061.096014
CCCGAGT65850.061.0214581
AAGTGCT312950.060.5126465
CTCGGGA82900.060.404035
AAAGTGC307700.060.403834
CCAAAGT326350.059.83172
AGTGCTG314250.058.896486
CCCAAAG360250.058.518331
Produced by FastQC (version 0.11.5)